BFV/Va Bovine
Factor V/Va
Purified from freshly collected
bovine plasma using a combination of salt precipitations and column
chromatography. This protein is a mixture of Factor V/Va and intermediate
activation products as judged by 4-20% gradient gels. Bovine Factor
Va functions as a cofactor with the serine protease Factor Xa in the
activation of prothrombin.
This protein may be used as a source of V/Va in the Prothrombinase
complex.
Buffer composition = 20
mM HEPES-HCl/0.15 M NaCl/pH 7.4/50% glycerol
*Extinction Coefficient (1%) = 9.6/17.4
One unit = 10 µg
Molecular Weight = 330,000; 94,000; 74,000; 71,000; 31,000 daltons
Concentration determined by BCA
Bovine Factor V/Va Sample Certificate of Analysis