HFIXa 1070 Human
Factor IXa α
Prepared
from Human Factor IX by activation with Russell's Viper Venom.
This RVV-X cleaves a single internal Arg-Val peptide bond in the Factor
IX. The RVV-X is removed after activation. Complete activation is
observed by SDS-PAGE. This protein purity is determined by
SDS-PAGE and shows total reduction upon incubation with
2-mercaptoethanol. This form of activated Factor IX has about 50% of
the coagulant activity of Human Factor IXaβ.
Buffer composition = 20 mM Tris-HCl/0.1 M NaCl /pH
7.4
*Extinction Coefficient (1%) =14.9
One unit = 5 µg
Molecular Weight = 56,000 daltons
Human Factor IXa Alpha Sample Certificate of Analysis