HFVIIa Human
Factor VIIa
Prepared from
purified Human Factor VII using Human Factor XIIa. The Factor Xlla is
removed using affinity chromatography. Purity is determined by
SDS-PAGE. Human Factor VIIa reduces to 29,500 and 23,500 with the
addition of 2-mercaptoethanol. Activity is determined via clotting
assay. Factor Vlla, in the presence of calcium ions and Tissue
factor, activates Factors IX and X to their enzymatically active forms,
Factor IXa and Xa.
Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4
*Extinction Coefficient (1%) = 13.9
Molecular Weight = 50,000 daltons
Also available: des-Gla-Human Factor VIIa and
Factor VIIa inactivated
Human Factor VIIa Sample Certificate of Analysis