HPKa 1303
Human Kallikrein
Activation of Prekallikrein with Factor α-XIIa
produces the enzymatically active Kallikrein. Kallikrein is a serine
protease which consists of a heavy chain (Mr 52kD) and light chains (Mr
either 36 or 33 kD) linked by disulfide bridges. Kallikrein possesses
enzymatic activity toward Factor XII, HK, Plasminogen, Factors XI, IX,
and VII, prorenin and the complement system. After activation, the
activating enzyme Factor XIIa is removed by affinity chromatography.
Human Kallikrein purity is determined by SDS-PAGE and shows complete
reduction upon incubation with 2-mercaptoethanol.
Buffer composition = 4
mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3
*Extinction Coefficient (1%) = 11.7
Molecular Weight = ~86,000 daltons
Also Available: Prekallikrein Activator
Human Kallikrein Sample Certificate of Analysis