Purified from fresh frozen
human plasma. AT is a single-chain glycoprotein which is considered
to be the main physiological inhibitor of thrombin and Factor Xa.
In the presence of heparin, the inhibition of thrombin and factor
Xa is enhanced from 300 to 1,000 fold. AT purity is >95% pure by
SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.
20 mM Tris-HCl/0.1 M Citric Acid/0.15 M NaCl/pH 8.3
*Extinction Coefficient (1%) = 6.5
Molecular Weight = 58,000 daltons
Human Antithrombin Sample Certificate of Analysis