Conversion of soluble fibrinogen
to the insoluble fibrin is the final step in the blood coagulation
cascade. ERL offers three types of purified fibrinogen prepared by
a combination of salt precipitation, affinity chromatography and immunoaffinity
chromatography methods to suit the individual researcher's needs.
All three forms are >95% clottable (by functional assays) and purity is >95% by SDS-PAGE.
- FIB 1 Plasminogen Depleted
- FIB 2 Plasminogen and von Willebrand Factor Depleted
- FIB 3 Plasminogen, von Willebrand Factor and Fibronectin Depleted
Buffer composition = 20 mM Sodium Citrate-HCl/pH 7.4
*Extinction Coefficient (1%) = 15.1
Molecular Weight = 330,000 daltons
Human Fibrinogen 1 Sample Certificate of Analysis
Human Fibrinogen 2 Sample Certificate of Analysis
Human Fibrinogen 3 Sample Certificate of Analysis
Also available: Peak '1' and Peak '2'
It has been
known for many years [Mosseson MW & Finlayson]S.] Lab Clin Med
62:663, 1963] that human fibrinogen can be sufractionated
chromatographically into two peaks called Peak '1' and Peak '2'
Fibrinogen respectively. These fibrinogens differ from one
another with respect to their gamma chain composition. Peak '1'
fibrinogen accounts for 85% of the total plasma fibrinogen and
has two gamma-A chains, whereas Peak '2' fibrinogen accounts for
the remaining 15% and contains one gamma-A chain and one gamma
chain per molecule. Peak '1' fibrinogen contains mostly intact
A-alpha subunit chains, is free of plasminogen, factor XIII,
fibronectin, and other non-fibrinogen protein contaminants. Peak
1' fibrinogen molecules each contain two copies of the
platelet-binding gamma-A chain C-terminal sequence and are ideal
substrates for factor XIII assay. Peak '2' fibrinogen contains
measurable amounts of factor XIII activity because the factor
XIII B subunits bind to gamma chains and bring catalytic A
subunits along with them. Thus Peak '2' fibrinogen serves as a
'carrier' for factor XIII in blood. The gamma chain lacks the
platelet recognition sequence but, like all gamma chains, become
crosslinked to dimers by factor XIIIa.
Peak '1' = 340,000 daltons
Peak '2' = 342,000 daltons
Peak '1' = 15.1
Peak '2' = 15.1
Clottability = > 95%
Peak '1' Purified Fibrinogen Sample Certificate of Analysis
Peak '2' Purified Fibrinogen Sample Certificate of Analysis