GP2b3a
Human GPIIbIIIa
Platelet membrane glycoproteins
are involved in platelet adhesion and aggregation. Glycoproteins IIb
and IIIa (GPIIb and GPIIIa) constitute the fibrinogen receptor and
are required for platelet aggregation. ERL offers GPIIbIIIa purified
from human platelets. Glycoprotein IIb consists of 2 disulfide-linked
subunits GPIIb (MW = 125,000) and GPII (MW= 23,000) while GPIIIa has
only one polypeptide chain (MW= 108,000). GPIIbIIIa migrates on gels
as follows: GPIIb 136,000 non-reduced and 125,000 reduced. GPIIIa
is 97,000 non-reduced and 108,000 reduced. Protein concentration is
determined via the Bradford method.
Buffer Composition = 20 mM Tris-HCl/0.1 M NaCl/0.1%
Triton X-100/
1 mM CaCl2/0.05% NaN3/50% glycerol/ pH 7.4
*Extinction Coefficient (1%) = 9.1
Human GPIIbIIIaSample Certificate of Analysis