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TECHNICAL
INFORMATION |
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Protein
Handling
Enzyme Research Laboratories, Inc. makes the following
suggestions for the storage and handling of purified proteins:
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- Most purified proteins
are stable at low temperatures and should be stored at
<-60°C.
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Lyophilized proteins should be reconstituted using a
good laboratory quality H2O. After reconstitution the
protein should be aliquoted into a useful (one time use)
size and stored at <-60°C.
- Freeze
proteins in a concentrated form and as soon as possible. When dilution is necessary,
dilute immediately prior to use. When possible,
dilution buffers should contain stabilizers such as albumin.
- Limit
thawing and refreezing. Multiple thaw/freezes can reduce
protein activity and cause degradation.
We always suggest aliquoting the protein into a single
use size.
- Refrigerate
thawed proteins or store on ice. Proteins warmed even to room
temperature may degrade quickly.
- One
exception to # 5 is Fibrinogen.
After thawing Fibrinogen, keep at room temperature.
Fibrinogen will come out of solution if it is cooled
- Products
provided in Glycerol/buffer solutions should be stored at
-20°C.
Temperatures below -30°C can cause phase transitions and
need to be avoided.
Unit Definition 1 PEU is equal to the amount of
activity found in one mL of plasma as determined in factor
deficient clotting assay. All extinction coefficients are reported for 280 nm using
1 cm path length.
Shipping
Information
Unless otherwise noted, all the coagulation factors are stored
at <-60°C
and shipped on dry ice by overnight carrier.
Shipping will be billed.
All
Human Source material is tested and found negative for all communicable
diseases, including HIV-1 and HIV-2 antibodies, HCV, Rapid Plasma
Reagin and Hepatitis B Surface Antigen. Because no test method
can offer complete assurance that Human Immunodeficiency Virus
(HIV), Hepatitis B virus or other infectious agents are absent,
all human proteins should be handled as suggested in the U.S.
Department of Health and Human Services Manual on Biosafety
in Microbiological & Biomedical Laboratories.
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©Enzyme
Research Laboratories, 2004
1801 Commerce Drive, South Bend, IN 46628
574.288.2268 or 800.729.5270 FAX: 574.288.2272 | | |
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